Multiplex Immunofluorescence 

Multiplex immunofluorescence (mIF) allows investigators to capture expression patterns of several markers in a single tissue section. Composite images integrate signals from up to 6 markers in a single 'stacked' picture.
The lab conducts automated mIF slide staining, acquires multispectral slide images, and uses advanced machine learning-based image analysis algorithms for cell segmentation and characterization.
  • Measure up to 6 tissue antigens simultaneously, plus a DAPI stain for DNA.
  • Use cell lineage markers to characterize heterogeneous tissues.
  • ​Spatially resolve patterns of marker co-expression or activation of cell signaling pathways in tissues.

Design a custom panel or add a marker of your choice to an already optimized 5-plex panel from Akoya Biosciences:
  • Immune profile panel: CD3, CD8, CD20, CD68, PanCK + one marker of your choice
  • Immunocontexture panel: CD8, CD68, PD-L1, FoxP3, PanCK + one marker of your choice
  • Activated Tumor Infiltrating Lymphocyte (TIL) panel: CD3, CD8, Ki67, Granzyme B, PanCK + one marker of your choice
  • M1/M2 polarization panel: CD8, CD68, PD-L1, PD-1, CD163 + one marker of your choice
  • T cell status panel: CD4, CD8, CD20, FoxP3, PD-1 + one marker of your choice

​Akoya Resources
www.akoyabio.com/phenocode-signature/
Phenocycler Fusion technology

The PhenoCycler-Fusion (formerly CODEX) platform works by (i.) staining tissue sections with multiple barcoded antibodies in a single step, and then (ii.) iteratively adding and imaging complementary oligos conjugated to fluorophores in "Reveal - Image - Remove" cycles. By automating the fluidics and imaging processes under isothermal conditions, PhenoCycler-Fusion enables labs to quickly generate highly multiplexed, high-resolution whole slide images. The instrument produces Gb-sized QPTIFF format files, compatible with many software packages for spatial data analysis.